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High-Throughput Protein Expression Screening

 

High-Throughput Expression Platform
  • The Facility has acquired a customized Tecan Evo system for high-throughput protein expression screning in 24-well plates. Various conditions like E. coli strain, lysis buffer and expression construct can be compared.

  • The Platform is completely automated and includes cell lysis (sonication), separation of insoluble and soluble fractions, affinity-purification of the over-expressed proteins and preparation of samples for SDS PAGE gel analysis. The process takes 2 to 3 hrs, starting from E.coli cell pellets obtained from the 24-well culture plates until the delivery of the final samples.  For purification purposes, the over-expressed proteins should contain a (His-) tag.

NKI Protein Facility                                                                                                                                                                                                         Last updated: 13 April 2012

NKI-AvL

Plesmanlaan 121

1066 CX Amsterdam

The Netherlands